Same procedures describes in in the experiment " Effect of live bacteria on A549 cells attachment (2 hours incubation) in a 96 well plates" but using a 24 well plates seeded with A549 cells and adding 1 mL of bacterial broth or bacterial supernatant to each well with 1 mL of RPMI media . after 3 hours incubation time , the attachment of the cells were measured suing MTT assay.
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