PCR for LVS chiT3 .

3/3/2015

Protocol 

95 c                    3 minutes

95 c                    30 second

41 c                     1.5 minutes                                35 cycle

68 c                    1.5 minute
Final elongation 5 minutes, total volume 25 microliter.

Samples:
1-chiT3

1.5 mico primares x 2
1.5
micro g DNA
20 micro supermix.
Run on 1% gel for 3 hours.

SOE PCR for clpB

clpB3 R primer 1.5 μL
clpB5 F primer 1.5 μL
supermix 21 μL


Protocol:
 95 °C    3 minutes

95 °C   30 seconds
55 °C  2 minutes                    30 cycles
68 °C  1.5 minutes

Final elongation 68 °C  for 5 minutes.

1% agarose run for 4 hours.