This blog is the ONS (Open Notebook Science) record for the work that I personally perform in the lab. It is posted informally and without peer review. Please feel free to comment and to contact me at osama.mahdi[at]my.ndsu.edu if there is something you're interested in. You can learn more about the lab on our wiki page at http://openwetware.org/wiki/Fisher. Thanks for visiting.
Friday, March 6, 2015
SOE PCR for clpB
clpB3 R primer 1.5 μL clpB5 F primer 1.5 μL supermix 21 μL Protocol: 95 °C 3 minutes 95 °C 30 seconds 55 °C 2 minutes 30 cycles 68 °C 1.5 minutes Final elongation 68 °C for 5 minutes. 1% agarose run for 4 hours.
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