SOE PCR for clpX

2/23/2015

3 PCR 1.5 μL (PCR product from clpX 3)
5 PCR 1.5  μL (PCR product from clpX 5)
clpX3 R primer 1.5 μL
clpX5 F primer 1.5 μL
supermix 21 μL


Protocol:
 95 °C    3 minutes

95 °C   30 seconds
55 °C  2 minutes                    30 cycles
68 °C  1.5 minutes

Final elongation 68 °C  for 5 minutes.

1% agarose run for 4 hours.