PCR for LVS clpX3, clpB5 and chiT5

2/2/2015

Protocol 3

95 c                    7 minutes

95 c                    30 second

49 c                     30 seconds                                  35 cycle

68 c                    1 minute

Final elongation 5 minutes, total volume 25 microliter.

Samples:1- clpX 3

1- clpX 3

2-clpB 5

3-chiT 5

Result:  clpX 3 showed band

Run on 1% gel for 3 hours.