2/8/2015
Protocol 4
95 c 3 minutes
95 c 30 second
46 c 30 seconds 35 cycle
68 c 1 minute
Final elongation 5 minutes, total volume 25 microliter.
Samples:
1- clpB3
2-chiT3
3-clpX5
4- clpB5
5- chiT5
1.5 mico primares x 2
2 micro g DNA
20 micro supermix.
Run on 1% gel for 3 hours.
1- clpB3
2-chiT3
3-clpX5
4- clpB5
5- chiT5
1.5 mico primares x 2
2 micro g DNA
20 micro supermix.
Run on 1% gel for 3 hours.
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