PCR for LVS chiT3, clpX3,, clpB5 and chiT5

2/11/2015

Protocol 5

95 c                    3 minutes

95 c                    30 second

44 c                     1.5 minutes                                35 cycle

68 c                    1.5 minute
Final elongation 5 minutes, total volume 25 microliter.

Samples:
1-chiT3
2-clpX3
3- clpB5
4
- chiT5
1.5 mico primares x 2
2 micro g DNA
20 micro supermix.

Run on 1% gel for 3 hours.