The protocol in the link
Plate name :collection of 3 paltes(contain 10 +20+10 colonies), pDR1/kana. transposon mutagensis.
The result: after 4 days incubation at room temp. in a dark place
Plaques in Kleb. plate=1 plaques in dilution 1/1000 (-3)
Plaque forming unit pfu =# plaque X dilution factor/volume of inoculum
=1.0 X10^3
2 μl of 8 Dicty. dilutions that contain(2.0 X 10^2, 2.0 X 10^1, 2 , 0.2,....) Dicty. cells were used on sm2 agar in four replicate for each dilution.
The result after 4 days :
Controls:
9-2A8 named: pDR1tn9
=1.0 X10^3
2 μl of 8 Dicty. dilutions that contain(2.0 X 10^2, 2.0 X 10^1, 2 , 0.2,....) Dicty. cells were used on sm2 agar in four replicate for each dilution.
The result after 4 days :
Controls:
- pDR1plague :(0)
- cosmid control.plague(0,-1,-2)
- Clones shows sensitivity to Dictyostelium predation:
9-2A8 named: pDR1tn9
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