Dictyostelium Predation Assay for transposon mutagensis of pDR2 Part(2)

Date: 10/1/2014
The protocol in the link
Plate name :  pDR 2/Kana. P1 & 4 , transposon mutagensis.
 The result: after 4 days incubation at room temp. in a dark place
 Plaques in Kleb. plate=1 plaques in dilution 1/1000 (-3)

Plaque forming unit pfu =# plaque X dilution factor/volume of inoculum
                                      =1.0 X10^3

2 μl of  8 Dicty. dilutions that contain(2.0 X 10^2, 2.0 X 10^1, 2 , 0.2,....)Dicty. cells were used on sm2 agar in four replicate for each dilution.
The result after 4 days :
Controls:

pDR2plague :( 0, -1, -2 )
 cosmid control.plague(0,-1,-2,-3)
Clones shows sensitivity to Dictyostelium predation:
plague (0,-1,-2,-3)