osama`s notebook

This blog is the ONS (Open Notebook Science) record for the work that I personally perform in the lab. It is posted informally and without peer review. Please feel free to comment and to contact me at osama.mahdi[at]my.ndsu.edu if there is something you're interested in. You can learn more about the lab on our wiki page at http://openwetware.org/wiki/Fisher. Thanks for visiting.

Friday, April 24, 2015

Plasmid minipreb for ClpX, ClpB and ChiT

4/17/2015

Clones:
ClpX 1,2,3,4
ClpB 1,2,3,4
ChiT 1,2,3,4
Clones fromTransformation using chemically competent cells for ClpX,ClpB and ChiT + pJH1

Zyppy™ Plasmid Miniprep Kit was used Protocol



Posted by osama mahdi at 12:37 PM
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Blog Archive

  • ▼  2015 (17)
    • ►  May (3)
    • ▼  April (7)
      • Plasmid minipreb for ClpX, ClpB and ChiT
      • SOE PCR for clpX, ClpB and ChiT
      • Transformation using chemically competent cells fo...
      • Ligation SOE PCR with pJH1
      • End repair for SOE (Clpx,ClpB and Chit) PCR.
      • Enzyme digest and treatment pJH1 with SmaI, and An...
      • Plasmid miniprep. pJH1
    • ►  March (2)
    • ►  February (4)
    • ►  January (1)
  • ►  2014 (23)
    • ►  December (3)
    • ►  November (3)
    • ►  October (5)
    • ►  September (1)
    • ►  August (3)
    • ►  July (5)
    • ►  June (2)
    • ►  May (1)

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