SOE PCR for clpX, ClpB and ChiT

4/10/2015

1-
1.5  μL ClpX 3`           sample 1,     2/2/2015
1.5  μL ClpX 5`           sample 2,     2/11.2015  
1.5  μL ClpX 3` R primares
1.5  ClpX 5` F primares
20 micro supermix.
2-
1.5  μL ClpB 3`           sample 1,     2/8/2015
1.5  μL ClpB 5`           sample 3,     2/11.2015  
1.5  μL ClpB 3` R primares
1.5  ClpB 5` F primares
20 micro supermix.

3-
1.5  μL ChiT 3`           sample ,     3/3/2015
1.5  μL ChiT 5`           sample 4,     2/11.2015  
1.5  μL ChiT 3` R primares
1.5  ChiT 5` F primares
20 micro supermix.

Protocol:
 95 °C    3 minutes

95 °C   30 seconds
55 °C  2 minutes                    30 cycles
68 °C  1.5 minutes

Final elongation 68 °C  for 5 minutes.