Dictyostelium Predation Assay for transposon mutagensis of pDR1

Date: Oct./17/2014
The protocol in the link
Plate 1, pDR1/kana. transposon mutagensis.
 The result: after 4 days incubation at room temp. in a dark place

Plaques in Kleb. plate

Plaque forming unit pfu =# plaque X dilution factor/volume of inoculum

Controls: pDR1 and cosmid control.
Clones shows sensitivity to Dictyostelium predation:
plague (0,-1,-2) 
1-B8 named: pDR1tn1

 plague(0,-1,-2,-3)
2-H6 named pDR1tn2
3-H8             pDR1tn3
plague(0,-1,-2,-3,-4)
4-H1            pDR1tn4
5-H2            pDR1tn5