Dictyostelium Predation Assay

Same procedure and protocol used in `Dictyostelium Predation Protocol(original)`.
Starting it on 10/7/2014 for the clones pDR 1,2,4,5,6 and cosmid contol.
DH5 alfa E coli
S.maltophilia BAA84
S.maltophilia N
S.maltophilia  M
S.maltophilia  H

The result: after 5 days incubation at room temp. in a dark place

Plaques in Kleb. plate

Plaque forming unit pfu =# plaque X dilution factor/volume of inoculum

Pfu = 8 X 10³ /.05 = 1.6 X 10⁴  

Bacterial strain	# of Dicty cells to form a plaque
Cosmid control	0.16
DH5 alfa E.coli	0.16
pDR1	1.6 X 104    =16000
pDR2	1.6
pDR4	1.6 X 102    =160
pDR5	1.6 X 102    =160
pDR6	1.6 X 102   =160
Steno.maltophilia  BAA84(D457)	1.6 X 104                           small plaques
Steno.maltophilia (N)	1.6 X 103                          small plaques
Steno.maltophilia(M)	1.6 X 103                          small plaques
Steno.maltophilia(H)	1.6 X 103                          small plaques