The protocol in the link
Plate name : individual colonies(contain 10 colonies), pDR1/kana. transposon mutagensis.
The result: after 4 days incubation at room temp. in a dark place
Plaques in Kleb. plate=32 plaques in dilution 1/1000 (-3)
Plaque forming unit pfu =# plaque X dilution factor/volume of inoculum
32 X10^3/10 = 3.2 X10^3
2 μl of 8 Dicty. dilutions that contain(6.4 X 10^3, 6.4 X 10^2, 6.4 X 10 ,6.4 , 0.64,....) Dicty. cells were used on sm2 agar in four replicate for each dilution.
The result after 4 days :
Controls:
8-A5 named: pDR1tn8
32 X10^3/10 = 3.2 X10^3
2 μl of 8 Dicty. dilutions that contain(6.4 X 10^3, 6.4 X 10^2, 6.4 X 10 ,6.4 , 0.64,....) Dicty. cells were used on sm2 agar in four replicate for each dilution.
The result after 4 days :
Controls:
- pDR1plague :(0,-1)
- cosmid control.plague(0,-1,-2,-3,-4)
- Clones shows sensitivity to Dictyostelium predation:
8-A5 named: pDR1tn8
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