The protocol in the link
Plate 4, pDR1/kana. transposon mutagensis.
The result: after 4 days incubation at room temp. in a dark place
Plaques in Kleb. plate=23 plaques in dilution 1/1000 (-3)
Plaque forming unit pfu =# plaque X dilution factor/volume of inoculum
23 X10^3/10 = 2.3 X10^3
2 μl of 8 Dicty. dilutions that contain(4.6 X 10^3, 4.6 X 10^2,4.6 X 10 ,4.6 , 0.46 ,....) Dicty. cells were used on sm2 agar in four replicate for each dilution.
The result after 4 days :
Controls:
6-A7 named: pDR1tn6 - small palques-
plague(0,-1,-2,-3,-4)
7-A6 named: pDR1tn6
The result after 5 days incubation look like same
23 X10^3/10 = 2.3 X10^3
2 μl of 8 Dicty. dilutions that contain(4.6 X 10^3, 4.6 X 10^2,4.6 X 10 ,4.6 , 0.46 ,....) Dicty. cells were used on sm2 agar in four replicate for each dilution.
The result after 4 days :
Controls:
- pDR1plague :(0,-1,-2)
- cosmid control.plague(0,-1,-2,-3,-4)
- Clones shows sensitivity to Dictyostelium predation:
6-A7 named: pDR1tn6 - small palques-
plague(0,-1,-2,-3,-4)
7-A6 named: pDR1tn6
The result after 5 days incubation look like same
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